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Ph Indicator
Acid Fuchsin
C.I. No. : 42685
C.I. Name : Acid Violet 19
CAS No. : 3244-88-0
Appearance : Dark Greenish Powder
Solubility (0.1 % in dil HCL) : Clear Red Solution
Absorptivity(A1% 1cm at lamda Max 547 nm in dil HCL : 1057
Dye Content : 81.00 Min
Empirical formula : C20H17N3Na2O9S3
Loss on drying at 110 deg C : 2.95 %
Bromocresol Purple
CAS No. : 115 - 40 - 2
Chemical Formula : C21H16Br2O5S
Molecular Weight : 540.24
Melting Point : 240° c
Dye Content : ~95%
Transition Range : pH 5.2 - 6.8 Greenish Yellow to Purple
Absorption Max(pH5.2) : 427 - 431 nm
Absorption Max(pH6.8) : 588 - 590 nm
Absorptivity(1%,1cm)pH 5.2 : 400 - 450
Absorptivity(1%,1cm)pH 6.8 : 1000 - 1100
Loss On Drying : <1%
Janus Green B
C.I.No : 11050
Chemical Name : Janus Green B
CAS No. : 2869-83-2
Chemical Formula : C30H31ClN6
Molecular Weight : 511.07 gm/mole
Dye Content (Spectrophotometry) : >65%
Absorption Max (50% Ethanol) : 664 +/- 13nm
Absorptivity (A1%/1cm., in 50% Ethanol) : > 550
Loss On Drying (110° c) : < 5%
Neutral Red
C.I.No : 50040
Chemical Formula : C15H17ClN4
CAS No. : 553-24-2
Molecular Weight : 288.78
Dye Content : ~60%
Absorption Max (EtOH 50%) : 539 - 544 nm
Absorptivity(1%,1cm) : >900 (on dry basis)
Transition Range : pH 6.8 - 8.0 Red - Orange
Loss On Drying (110° c.) : < 8%
Leishman's Stain
CAS No. : 12627-53-1
Appearance : Green Crystalline Powder
Solubilty 0.1%(MeOH) : Clear Blue Solution with Green Fluorescence
Absorptivity 1% 1 Cm lamda 524.0 nm lamda 647.0 nm : 856 ( >650) 1192 (>900)
Loss on Drying : 6.42% (< 8%)
Alizarin Red S
C.I.No : 58005
C.A.S No. : 130-22-3
Class : Anthraquinone
Solubility in 0.1% water : Clear Red Soln
Absorptivity 1% 1 cm :
(pH 6.3) at lamda max 421.5 nm : 150
(pH 9.3) at lamda max 525.5 nm : 288
Loss on drying : 3.58 %
Malachite Green
C.I.No : 42000
C.A.S No. : 2437-29-8
C.I. Name : Basic green 4
Appearance : Green Crystals
Solubility 0.1% (water) : Clear Solution
Absorptivity 1% ,1cm at lamda 617.0 nm : 1858 (limit > 1730)
Loss on Drying : 4.25 %
Empirical formula : C23H25N2Cl
Formula weight : 365
METHYL ORANGE
C.I.No : 13025
C.A.S No. : 547-58-0
Chemical Formula : C14H14N3NaO3S
Molecular Weight : 327.34
Melting Point : 300° c
Dye Content : ~95%
Transition Range : pH 3.0-4.4 Pink-Orange yellow
Absorptivity(pH 3.0)501-504nm : 1050-1150
Absorptivity(pH 4.4)467-471nm : 750-850
Loss On Drying (110 ° c) : < 5%
Azure 1(B)
C.I.No : 52010
Common name : Azure B
Suggested name : Azure B
Solubility in water : Soluble
Solubility in ethanol : Sparingly
Absorption maximum : 648-655
Colour : Blue
Empirical formula : C15H16ClN3S
Formula weight : 305.83
Azure A
C.I.No : 52005
Common name : Azure A
Suggested name : Azure A
Solubility in water : Soluble
Solubility in ethanol : Sparingly
Absorption maximum : 620-634
Colour : Blue
Empirical formula : C14H14ClN3S
Formula weight : 291.8
Basic Fuchsin
C.I.No : 42510
C.A.S. No. : 632 - 99 - 5
Chemical Formula : C20H20ClN3
Molecular Weight : 337.86
Dye Content : ~88%
Absorption Max : 549 - 552 nm
Absorptivity(1%,1cm) : 2350 - 2700
Loss On Drying : < 10 %
Bromo Phenol Blue
C.A.S. No. : 115 - 39 - 9
Chemical Formula : C19H10Br4O5S
Molecular Weight : 669.98
Melting Point : 272 - 273° c
Dye Content : ~95%
Transition Range : pH 3.0 - 4.6 Greenish Yellow - Reddish Blue
Absorption Max (pH3.0) : 434 - 439 nm
Absorption Max (pH4.6) : 590 - 593 nm
Absorptivity (1%,1cm) pH 3.0 : 350 - 385
Absorptivity (1%,1cm) pH 4.6 : 940 - 1000
Loss On Drying (110° c) : <1%
Methylene Blue Zinc Free
C.I. No. : 52015
Appearance : Dark Green Crystalline Powder
Assay ( as is Basis ) : 85%
Assay ( on dry Basis ) : 99%
Loss on drying : 10.25%
Solubility : Clear Blue Solution
Ash Content : 0.74%
Residual Solvent : Nil
Aluminium : 100ppm
Cadmium : Not Detected
Chromium : 10 ppm
Copper : 50 ppm
Tin : 10 ppm
Iron : 100 ppm
Mangenese : 3 ppm
Mercury : Nil
Molybdenum : 10 ppm
Absorptivity 1%, 1 cm, at ^max.663.0nm : 2284
Methyl Red
CAS No. : 493-52-7
C.I.No. : 13020
Chemical Formula : C15H15N3O2
Molecular Weight : 269.31
Melting Point : 179-182° c
Dye Content : ~95%
Absorption Max(pH4.5) : 523-526 nm
Absorption Max(pH6.2) : 430-434 nm
Absorptivity(1%,1cm)pH 4.5 : >1330
Absorptivity(1%,1cm)pH 6.2 : >700
Transition Range : pH 4.2 - 6.2 Red - Yellow
Loss On Drying (110 ° c) : < 5%
Orange G
CAS No. : 1936-15-8
C.I.No. : 16230
Appearance : Orange Powder
Solubility 0.1 % (water) : Clear Orange Solution
Absorptivity 1% 1cm at Lamda max 478.0 nm : 500 ( limit 470-520)
Loss on Drying : 3.57 %
Empirical formula : C16H10N2O7S2Na2
Formula weight : 452.386
Bromothymol Blue
CAS No. : 76 - 59 - 5
Chemical Formula : C27H28Br2O5S
Molecular Weight : 624.40
Melting Point : 200 - 202° c
Dye Content : ~95%
Solution Clarity : To pass
Transition Range : pH 5.8 - 7.6 Yellow - Blue
Absorption Max (pH 5.8) : 430 - 435 nm
Absorption Max (pH 7.6) : 615 - 618 nm
Absorptivity (1%/1cm) pH 5.8 : 260 - 300
Absorptivity (1%/1cm) pH 7.6 : 470 - 520
Loss On Drying (110° c) : < 3%
Phenol Red
CAS No. : 143-74-8
Chemical Formula : C19H14O5S
Molecular Weight : 354.38
Dye Content : ~95%
Solution Clarity (Water) : To pass
Transition Range : pH 6.5 - pH 8.0
Absorptivity (pH 1.2) 503 - 506 nm : 1000 - 1100
Absorptivity (pH 3.0) 430-435 nm : 600-700
Absorptivity (pH 6.5) 430 - 435 nm : 600 - 700
Absorptivity (pH 8.0) 557 - 560 nm : 1100 - 1200
Loss On Drying (110° c) : < 1%
Phenol Red Sodium Salt
CAS No. : 34487-61-1
Chemical Formula : C19H13NaO5S
Molecular Weight : 376.36
Melting Point : 285° c
Dye Content : ~90%
Solution Clarity (Water) : To pass
Transition Range : pH 6.5 - pH 8.0 Yellow to Red violet
Absorptivity (pH 1.2) 503 - 506 nm : 880 - 950
Absorptivity (pH 3.0) 430-435 nm : 560 - 620
Absorptivity (pH 6.5) 430 - 435 nm : 560 - 620
Absorptivity (pH 8.0) 557 - 560 nm : 970 - 1100
Loss On Drying (110° c) : < 5%
Chlorophenol Red
CAS No. : 4430-20-0
Chemical Formula : C19H12Cl2O5S
Molecular Weight : 423.28
Dye Content : ~70%
Solution Clarity (EtOH) : To pass
Transition Range : pH 4.8 - pH 6.4
Absorptivity (pH 4.8) 432 - 436 nm : 450 - 600
Absorptivity (pH 6.4) 574 - 578 nm : 1000 - 1300
Loss On Drying (110° c.) : < 8%
Diethyl Safranine
C.I.No. : 50206
CAS No. : 4569-86-2
Chemical Name : 3-Amino-7-(diethylamino)-5- Phenyl Phenazinium Chloride.
Chemical Formula : C22H23N4CI
Molecular Weight : 378.91 gm/mole
Dye Content (Spectrophotometre) : =>80%
Absorption Max (in water) : = 552 - 557 nm
Absorptivity (A1%/1cm. in water,) : = > 950
Loss On Drying (110° c) : = < 5%
Safranine O
CAS No. : 477-73-6
C.I.No. : 50240
Chemical Formula : C20H19ClN4
Molecular Weight : 350.85
Dye Content : ~95%
Absorption Max (EtOH 50%) : 530 - 534 nm
Absorptivity (1%,1cm) : > 1350 (on dry basis)
Loss On Drying (110° c) : 8% max
Nitrazine Yellow
Product No. : NTY 09394
Product Brand : NILE
Batch No. : 118/41
Molecular Weight : 542.36
TEST SPECIFICATION RESULTS
Appearance (Colour) Brown - Yellowish Orange Powder Complies
Appearance (Form) Powder
Wavelength 587 - 591 nm Complies
Extinction Coefficient >=35000
Dye Content 85% Complies
Solubility (Colour) Clear Yellow to Reddish Brown At PH 6.0 Bright Yellow
c=1 mg/ml; Water At PH 7.2 Bright Blue
Solubility (Turbidity) Clear Complies
Fluorescein
Specification : Lot : NC/2905
Batch Size : 250 Kgs.
C.I. No. : 45350
CAS No. : 2321-07-5
C20h12O5
Minimum Assay : 98.5%
Absorption Max : 491-496 nm
Loss on Drying : 8%
Maximum Limits of Impurities
Lead (Pb) : 0.002%
Copper (Cu) : 0.01%
Cadmium (Cd) : 0.001%
Zinc (Zn) : 0.01%
Arsenic (As) : 0.0002%
Acriflavine : Passes Test
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